Siming Ma

4 Questions 6 Answers 0 Followers

Questions related from Siming Ma

Can any one give samples in which viruses induce cellular protein translocation from cytoplasm to nucleus or reverse?

I am trying to write a summary about this before I start my new project, do not have enough clue and need to some ideas!

01 August 2017 7,773 22 View

Issues about Subcellular Protein Fractionation Kit for Cultured Cells by ThermoScientific - cytoskeletal fraction

Has anyone used the Subcellular Protein Fractionation Kit for Cultured Cells by ThermoScientific? I am very confused about the last fraction in this kit, which indicated as "cytoskeletal". But...

25 May 2017 6,021 1 View

Can anyone help me to find out what is going on to my plasmid digestion on agarose gel?

I am trying to do a routine cloning with GFP vector, 5500bp. I need to double digest it with SalI and BglII. I tried double digestion in NEB3.1, individual digest with each enzyme for 1hr then...

25 July 2016 2,679 9 View

Is there a problem to use lentivirus to infect HeLa cell line?

I had successful experience using lentivirus to infect 293T and ThP-1 cell lines. I am recently using pEasiLv system infecting HeLa cell but never got a single positive cell. I repeated 3 times...

14 July 2016 4,017 1 View