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I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
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Is it really important to know the Splicing sites position (5 prime or 3 prime)? As we already know, one possible reason might be that we can know from which region of the gene the introns were...
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