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Hello everyone, Recently, I have encountered a problem in cell culture. I use Rpmi 1640. I have freezed SKBR-3 cells 8 months ago. I am defreezing them now. I defreezed two cryovials up to now and...
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Hi all I'm working with qPCR and I persistently find 2 peaks in my qPCR analysis. While my PCR target is 127bps, I see a peak at 82°C and a sharp peak at 88°C. I checked it by agarose...
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