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Questions related from Sima Ravaei
Hi dear friends; I have encountered a very serious challenge these days about primer designing for mature miRna gene and I'm seeking help from you right now. I have designed a stem loop primer and...
23 May 2025 9,954 5 View
Hi guys I'm going to extract RNA from cell culture and I have to put RNA-containing microtubes in the water bath for 10 minutes at 60°C. I want to ask if any Rnase contamination danger threaten...
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Hello everyone, Recently, I have encountered a problem in cell culture. I use Rpmi 1640. I have freezed SKBR-3 cells 8 months ago. I am defreezing them now. I defreezed two cryovials up to now and...
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Hi guys, I want to ask if anyone knows any websites or bioinformatics resources where I can find MicroRNAs or lncRNAs interact with different genes in the shape of a pathway in cancers. I see new...
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Hi all I'm working with qPCR and I persistently find 2 peaks in my qPCR analysis. While my PCR target is 127bps, I see a peak at 82°C and a sharp peak at 88°C. I checked it by agarose...
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