Sima Ravaei

5 Questions 11 Answers 0 Followers

Questions related from Sima Ravaei

I have designed primers according to semloop method and the pcr product size is around 120 bp. It must be around 50-60 bp. what actions should I take?

Hi dear friends; I have encountered a very serious challenge these days about primer designing for mature miRna gene and I'm seeking help from you right now. I have designed a stem loop primer and...

23 May 2025 9,966 5 View

Water bath for rna extraction?

Hi guys I'm going to extract RNA from cell culture and I have to put RNA-containing microtubes in the water bath for 10 minutes at 60°C. I want to ask if any Rnase contamination danger threaten...

14 September 2024 3,420 5 View

Cell culture contamination?

Hello everyone, Recently, I have encountered a problem in cell culture. I use Rpmi 1640. I have freezed SKBR-3 cells 8 months ago. I am defreezing them now. I defreezed two cryovials up to now and...

23 May 2024 8,322 4 View

How to find MicroRNA - gene pathways in bioinformatics?

Hi guys, I want to ask if anyone knows any websites or bioinformatics resources where I can find MicroRNAs or lncRNAs interact with different genes in the shape of a pathway in cancers. I see new...

17 December 2022 4,890 3 View

why do I see one single band while I have 2 peaks in qPCR?

Hi all I'm working with qPCR and I persistently find 2 peaks in my qPCR analysis. While my PCR target is 127bps, I see a peak at 82°C and a sharp peak at 88°C. I checked it by agarose...

30 July 2021 9,829 8 View