Silke Werth

3 Questions 20 Answers 0 Followers

Questions related from Silke Werth

How can we do restriction digests of genomic DNA in the presence of inhibitors?

We would like to digest genomic DNA. Our restriction enzymes APE K1 and BAM H1 will cut lambda DNA, but will not cut our genomic DNA samples, even if we clean them up with several methods after...

10 June 2014 8,597 6 View

How can I optimize DNA binding to silica columns?

I am trying to isolate DNA using a CTAB based lysis buffer and a guanidine hydrochloride based binding buffer at a pH of 6. Final concentrations: guanidine hydrochloride 1.73M potassium acetate...

18 January 2013 4,419 3 View

How to optimize DNA binding to silica columns?

I am trying to isolate DNA using a CTAB based lysis buffer and a guanidine hydrochloride based binding buffer at a pH of 6. Final concentrations: guanidine hydrochloride 1.73M potassium acetate at...

18 January 2013 9,546 16 View