Sifan Wang

6 Questions 17 Answers 0 Followers

Questions related from Sifan Wang

What is the meaning of subscript numbers in the upstream of a promoter?

Can anybody tell me what is the meaning of the numbers in the attached picture? The picture is the upstream of a promoter.

05 May 2013 1,754 11 View

Does the distance between RBS and ATG (initiation codon) effect protein expression?

I constructed a plasmid DNA part through standard BioBrickTM assembly and sent for sequencing. The sequencing result is a little different than with other previous parts I have constructed. The...

05 May 2013 447 2 View

Remove two restriction sites by PCR?

My target gene contains two PstI sites. Is it possible to remove them through PCR? Do I need to run PCR twice or just once? My target gene was cloned into a vector, more specifically, it is a...

04 April 2013 9,414 10 View

Concentration of glycerol used for storage of bacteria?

I looked up many protocols for freezing bacteria, but the concentration of glycerol varies from 20% to 80%. How should I choose? Is it v/v or w/w? One of the protocols mentioned spinning the cell...

04 April 2013 9,776 88 View

Does the speed of translation possibly influence the configuration of a protein?

The number of tRNA varies among different cells, which I think might cause a different speed of translation. I'm curious as to whether the speed will possibly have an impact on the configuration...

04 April 2013 3,465 11 View

Some questions about site-directed mutagenesis (SDM).

I have a plasmid containing the target gene with an unwanted PstI restriction site. I managed to do SDM to eliminate it. The first question is: is there a need to consider the codon usage bias...

04 April 2013 2,655 14 View