Sifan Wang

5 Questions 11 Answers 0 Followers

Questions related from Sifan Wang

Does the distance between RBS and ATG (initiation codon) effect protein expression?

I constructed a plasmid DNA part through standard BioBrickTM assembly and sent for sequencing. The sequencing result is a little different than with other previous parts I have constructed. The...

21 May 2013 8,045 1 View

Does the speed of translation possibly influence the configuration of a protein?

The number of tRNA varies among different cells, which I think might cause a different speed of translation. I'm curious as to whether the speed will possibly have an impact on the configuration...

09 April 2013 9,264 10 View

Remove two restriction sites by PCR?

My target gene contains two PstI sites. Is it possible to remove them through PCR? Do I need to run PCR twice or just once? My target gene was cloned into a vector, more specifically, it is a...

09 April 2013 5,984 9 View

Concentration of glycerol used for storage of bacteria?

I looked up many protocols for freezing bacteria, but the concentration of glycerol varies from 20% to 80%. How should I choose? Is it v/v or w/w? One of the protocols mentioned spinning the cell...

09 April 2013 2,586 87 View

Iron fortificaion; gene-related technique or food fortification?

I am thinking about iron fortification in rice. After some reading, I find many research are focus on genetic way. By genetically modification of rice, they manage to improve the iron...

27 March 2013 1,260 6 View