Shyam Kokkattunivarthil Uthaman

3 Questions 6 Answers 0 Followers

Questions related from Shyam Kokkattunivarthil Uthaman

MAb detecting two viral proteins?

Hi, What are the chances that the developed MAb can detect two viral proteins simultaneously? The MAbs were raised against purified viral particles. During the characterization studies, some...

29 May 2020 4,168 1 View

Why an unexpected single band in SDS-PAGE of purified IgG from ascitic fluid using AbFRONTIER Protein G Sepharose Fast Flow column?

I have tried to purify the IgG from mouse ascitic fluid using Abfrontier Protein G Sepharose fast flow column according to manufacture's protocol. The nanodrop reading is showing concentration of...

15 May 2018 3,781 7 View

What is the difference between a primer used in the SYBR green based real time PCR and a TaqMan probe, if both are targeting a single gene?

Whether the same primer sequence (Used in the SYBRG-based qPCR) can be used to prepare TaqMan probe, if they are specifically amplifies the target? If not, what would be the exact difference or...

12 December 2017 437 5 View