Shuoyang Liu

6 Questions 1 Answers 0 Followers

Questions related from Shuoyang Liu

What is the relationship between LPS type and LPS-induced Treg expansion in sepsis ?

Dear friends, Recently, we use LPS to induce 24h-mouse spesis model to explore the effect of some drugs to splenic Treg expansion. However, sometimes we failed to induce sepsis and splenic Treg...

03 March 2019 4,064 1 View

How to make mouse colon single cell suspension and preserve these cells for flow cytometry?

hi, guys. I am treat dss-induced mouse colitis model with anti-inflammation herbs now and I need to check the M1/M2 polarization in the inflammation site of the colon. May I know how to make mouse...

09 September 2018 459 0 View

May I know what is the softare for cytokine analysis by mouse inflammation kit?

Hi, guys. I think I met a problem now because my supervisor asked me to change from ELISA into Mouse,Inflammation,Kit,RUO - 552364 to test several cytokine at the same time. Could you please...

06 June 2018 6,855 3 View

Why I get low level of mature M1 ratio in spleen of DSS-induced mouse colitis model?

Hi, guys, I did DSS-induced colitis model recently to find whether my targeted herb has the anti-inflammatory or immunomodulatory function in inflammation mouse model. However, I find the ratio of...

06 June 2018 1,976 0 View

How many Tregs or T cells should be sorted for 3-5 group WB?

Hi, guys, I think I meat some problems now. A reviewer asked us to make Western blot to show MAPK and NF-kB pathway upon drug treated Tregs. He said this is doable since it requires only few...

04 April 2018 1,135 1 View

About the CFSE protocol

Dear guys: I will start the CFSE Flow cytometry for Treg proliferation in next month. However, I am totally unfamiliar with the related parameters and cells number. Could you please recommend...

11 November 2016 541 6 View