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Dear friends, Recently, we use LPS to induce 24h-mouse spesis model to explore the effect of some drugs to splenic Treg expansion. However, sometimes we failed to induce sepsis and splenic Treg...
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hi, guys. I am treat dss-induced mouse colitis model with anti-inflammation herbs now and I need to check the M1/M2 polarization in the inflammation site of the colon. May I know how to make mouse...
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Hi, guys, I did DSS-induced colitis model recently to find whether my targeted herb has the anti-inflammatory or immunomodulatory function in inflammation mouse model. However, I find the ratio of...
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Hi, guys, I think I meat some problems now. A reviewer asked us to make Western blot to show MAPK and NF-kB pathway upon drug treated Tregs. He said this is doable since it requires only few...
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Dear guys: I will start the CFSE Flow cytometry for Treg proliferation in next month. However, I am totally unfamiliar with the related parameters and cells number. Could you please recommend...
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