Shukkoor Kondengaden

4 Questions 8 Answers 0 Followers

Questions related from Shukkoor Kondengaden

How can we purify the PCR product using DNA capture probes?

We have synthesized a biotin tagged DNA capture probe for purifying the complementary ssDNA from a PCR reaction mix. When we tested the probe with the pure synthetic ssDNA is good, but when we...

16 May 2017 7,526 1 View

How can get ride off NTC amplification in Synthetic ssDNA qPCR ?

We are trying to do qPCR with the following template and primer set, GG CCT CCT CCA ATT AAA GAA ACG CAA CTT TAG AAA AGA AAA CGA TCT AAG CTC TAC GAT GTA AGA GTA CCC AAA TAA CGG TTC AC is the...

17 November 2016 2,809 4 View

QPCR of synthetic DNA; It works well with the PCR but no Ct in qPCR , any suggestions welcome ?

Hello, GG CCT CCT CCA ATT AAA GAA ACG CAA CTT TAG AAA AGA AAA CGA TCT AAG CTC TAC GAT GTA AGA GTA CCC AAA TAA CGG TTC AC is the template Tm = 70 FW primer: GGC CTC CTC CAA TTA AAG AA RV primer:GTG...

04 November 2016 4,872 8 View

How to design qPCR primers for a 75 bp synthetic ssDNA?

I am a synthetic/medicinal chemist. We are currently doing a DNA encoded library of small molecules, we plan to label each small molecule with about 40 bp ssDNA. Now I can add few bp for primers...

28 September 2016 2,085 1 View