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Questions related from Shujun Zhang
Hi all, I'm going to use Pierce mitochondrial isolation kit (cat no: 89874) to separate cytosol protein and mitochondrial protein for WB. 1. For lysis of mitochondrial, instructions provided...
06 June 2016 4,803 1 View
I'm using z-VDVAD-FMK and z-IETD-FMK to blcok the caspase 2 and 8 activation (50uM for 7h including 1h of pretreatment). But I found both inhibitors also inhibit the caspase 3/7 activity...
05 May 2016 7,501 5 View
We usually use TUNEL to detect apoptotic cell death before. But in macrophages, we found many tiny signals out of cells after LPS treatment. We consider that might be caused by necrosis or...
04 April 2016 4,962 1 View
Hi all I found that my target gene expression (consistent in mRNA level and protein level) is different in a cell line against different stimuli. So I want to know why this phenomenon...
04 April 2016 3,527 3 View
Recently, I detected some proteins in THP1 and primary monocytes after LPS (from 100g/ml to 1000g/ml) treatment for 24h, but the results seem unstable. Sometimes I can see dramatically decrease,...
03 March 2016 6,243 2 View