Shujun Zhang

5 Questions 10 Answers 0 Followers

Questions related from Shujun Zhang

Dose anybody know about Pierce mitochondrial isolation kit?

Hi all, I'm going to use Pierce mitochondrial isolation kit (cat no: 89874) to separate cytosol protein and mitochondrial protein for WB. 1. For lysis of mitochondrial, instructions provided...

06 June 2016 4,799 1 View

Can z-VDVAD-FMK and z-IETD-FMK blcok the caspase 3/7 activity?

I'm using z-VDVAD-FMK and z-IETD-FMK to blcok the caspase 2 and 8 activation (50uM for 7h including 1h of pretreatment). But I found both inhibitors also inhibit the caspase 3/7 activity...

05 May 2016 7,498 5 View

Could someone recommend a Annexin-V reagent or kit for adherent cells using fluorescent microscope without digestion?

We usually use TUNEL to detect apoptotic cell death before. But in macrophages, we found many tiny signals out of cells after LPS treatment. We consider that might be caused by necrosis or...

04 April 2016 4,958 1 View

How can I start to investigate mechanisms of the regulation of a specific gene?

Hi all I found that my target gene expression (consistent in mRNA level and protein level) is different in a cell line against different stimuli. So I want to know why this phenomenon...

04 April 2016 3,523 3 View

Do I need cell synchronization when detect proteins alteration in monocytes (THP1 cell line and primary) stimulated by LPS?

Recently, I detected some proteins in THP1 and primary monocytes after LPS (from 100g/ml to 1000g/ml) treatment for 24h, but the results seem unstable. Sometimes I can see dramatically decrease,...

03 March 2016 6,240 2 View