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I have been trying to perform phage purification (CsCl density centrifugation), DNA extraction (Norgen phage DNA isolation kit) and phage sequencing (MiSeq PE-150) for therapeutic phages targeting...
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Other than sequencing to check for presence of integrases, how do we experimentally determine if a phage is lytic or lysogenic?
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I isolated phage from river water using Klebsiella pneumoniae. During spot assay (spot on bacterial lawn), it gave a clear plaque lysis. However, when I tried plaque assay (mix soft agar with...
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I have observed lysis plaques on ATCC P. aeruginosa 27853 bacterial lawn prepared from overnight bacterial cultures. The bacterial strain is reported to contain prophage sequences in its genome....
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