Shubhendu Palei

2 Questions 9 Answers 0 Followers

Questions related from Shubhendu Palei

If acetonitrile can be converted to acetic acid in acidic hydrolysis conditions, then how is it widely used as a solvent for HPLC in a low pH?

In principle ACN should also hydrolyse in HPLC conditions, but it does not ! Is it not very strange and contradicting ??

05 August 2013 8,062 0 View

Does anybody have an idea of how to purify hydrophobic peptides by HPLC which are hard to solublize in initial HPLC conditions?

My peptide is hard to soluble in acetonitrile/water/0.5 % TFA solvent for preparative HPLC purification. I also tried in 2 mM NH3, but still it is very less soluble.

28 May 2013 6,182 20 View