Shubhanshu Pandey

3 Questions 2 Answers 0 Followers

Questions related from Shubhanshu Pandey

What is the concept of library Preparation for sequencing with sscircDNA tmepelate?

I have been trying to prepare a PCR library after doing the ChIP-Nexus protocol. After ligating the adapters and doing the required reaction (barcode filling on opposite strand and digesting the...

05 July 2024 1,192 0 View

What is happening to S2 cells?

I am trying to thaw S2R+ cells from the frozen culture. The cells (1 ml of cell culture) were frozen at passage 9 and stored at -80. I followed the steps below to thaw the cells from the frozen...

03 July 2023 4,252 4 View

How to estimate cell count of adherent cells on cover slips using fluorescent marker?

Hello, I am trying to do a cell count for S2 cells from Drosophila. These are more or less adherent cells and I have to do a kill curve using puromycin. I did a trial run where I grew the cells...

15 May 2023 516 0 View