Shubhabrata Majumdar

3 Questions 1 Answers 0 Followers

Questions related from Shubhabrata Majumdar

Do murine T-cells get transient activation when they attach to a tissue culture treated plate?

I have been trying to activate CD19 CAR-T cells using plate-bound anti-CAR Abs. I am getting activation both in cell lines and primary CD8 T-cells. I am using pErk as a readout for activation. I...

31 December 2020 1,770 2 View

What is a good time point after TCR stimulation to detect p65 phosphorylation in murine T-cells ?

I am trying to quantify phosphorylation of the p65 subunit of NF-kB in primary murine CD8+ T-cells. I stimulated the cells with plate-bound anti-CD3 (1ug/ml) and anti-CD28 (2ug/ml) Abs for 5min...

28 December 2020 1,214 2 View

How does laser power and duration of illumination impact tissue damage and inflammation?

I am trying to use a 405nm laser to photo-convert different fluorescent murine tissues to track migration of immune cells. I want to avoid tissue damage and inflammation with my laser which might...

23 February 2020 8,971 2 View