Shu Shuangshuang

2 Questions 3 Answers 0 Followers

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How to decide the efficiency of the medthod of intrarenal lentiviral gene delivery to knock down the target gene?

I want to konck down the target gene in mouse kidney. The method I want to take is recombinant lentivirus vectors harboring a short-hairpin RNA sequence are delivered to the mouse kidney by means...

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Why the siRNA could reduce mRNA expression but not protein expression?

Recently, I want to knockdown the expression of one gene with siRNA. There are four siRNA sequence targeting the gene. They can silence target gene expression by at least 75% at the mRNA level,...

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