Shruti Balaji

5 Questions 11 Answers 0 Followers

Questions related from Shruti Balaji

Using islets immediately upon isolation?

Hello. Our incubator for primary cultures is broken but I need to isolate some islets for both qPCR and estimating the insulin content. Has anyone performed either of these techniques on freshly...

11 November 2019 8,608 3 View

Any advice on the EtBr staining of protein in EMSA gel?

Hello. I'm a newbie at EMSA and am trying to get this to work for me. So I carry out my reaction, load &run the gel and the stain it with ethidium bromide in order to stain the DNA. However,...

09 September 2015 5,482 9 View

Positive signal only at low protein concentration?

I carried out a dot blot for various concentrations of my cell lysate and found weak positive staining for my protein of interest at a concentration of 15-25ng. However there was absolutely no...

12 December 2013 5,641 6 View

Brown colour precipitate in POD-conjugated antibody

I just took out a POD-labeled antibody from the frost-free -20oC where I had kept it last summer (so it is a year old now). It has a brownish tinge to it and I can't remember if it was colourless...

12 December 2013 4,866 2 View

Stripping PVDF membrane

I have a PVDF membrane with the proteins blotted onto it. I placed the membrane in blocking solution (2.5% non fat milk) overnight but I don't have the right antibody to probe it with. Can I just...

11 November 2012 7,520 10 View