Shrikant Pradhan

4 Questions 10 Answers 0 Followers

Questions related from Shrikant Pradhan

Why am I getting signal "overflow" with Pierce Renilla-Firefly Luciferase assay kit?

We recently tried the Pierce Renilla-Firefly luciferase kit. As mentioned in the product catalog, I set the detection of signals at 640nm for firefly followed by 525nm for Renilla luciferase....

09 March 2018 5,605 3 View

Can Matrigel be used for 3D cell culture studies?

Can spheroid formation of glioblastoma and breast cancer cells be induced using matrigel?

27 April 2016 3,502 3 View

Is it okay for LB agar plates and similar bacterial media to be exposed to UV?

I have often seen many of my colleagues put their media plates (mostly LB agar plates) along with the usual tip boxes and pipettes and switch on the UV for 15-20 minutes before starting their...

24 February 2014 1,163 3 View

What internal controls can be used while comparing the gene expression in normoxic versus hypoxic conditions?

I am working on lung cancer cells lines and comparing the expression of certain set of genes in normoxic versus hypoxic conditions.

27 December 2013 389 6 View