Shravasti Misra

3 Questions 2 Answers 0 Followers

Questions related from Shravasti Misra

Does anyone know whether the antibody BG4 against G quadruplexes bind both DNA and RNA G quadruplexes?

In the attached paper (by Shankar Balasubramaniam's group) they have used the BG4 antibody to pull down G4 RNA. Does this mean the antibody binds both DNA and RNA, only one with less affinity? Can...

22 February 2023 7,554 3 View

Is there a reason RNAse treatment, during immunocytochemistry, is done in Tris buffers and not PBS?

For a certain antibody staining I am doing a RNAse treatment, but the protocols usually mention doing this treatment in Tris-HCl buffers containing NaCl and usually a chelator like EDTA or sodium...

06 December 2022 5,897 2 View

After transformation of Dh5alpha competent cells I found a cloudy white ppt before plating. Can anyone explain this?

After transformation of Dh5alpha cells by heat shock I grew them at 37 in 200microlitre Terrific Broth (because I had it at hand) and then before plating I found cloudy white ppt in the cell...

04 February 2021 8,189 3 View