Shorok Sebaa

2 Questions 1 Answers 0 Followers

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Why are my negative controls (PBMCs and SUPT1 cells) giving positive HIV signal in ddPCR?

I am doing ddPCR for HIV targets (env, gag/LTR and 5'pol) with infected PBMCs. I am using uninfected PBMCs, SUPT1 cell lines as a negative control. I suddenly started having signal in my negative...

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In which components(evaporator,pump,...) can i charge feron in organic rankine cycle?

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