Shoaib Iqbal

3 Questions 7 Answers 0 Followers

Questions related from Shoaib Iqbal

Removal of PDMS from glass bonded chips

Hi, I wonder if bonding of PDMS to a glass can be reversed. I bonded PDMS to a glass slide using O2 plasma. After initial experiments, my device is not suitable for its intended application. So I...

08 August 2017 5,931 5 View

Unable to express recombinant antibody fragment?

Hi, I’m expressing a recombinant antibody fragment (ScFv) with C-terminal 6x His-tag. The protein is cloned in pET28a vector and is 29 kDa in size. I tried cell strains like Transetta (DE3) and...

11 November 2016 6,136 7 View

Why are RAW 264.7 cells treated first followed by LPS activation to measure treatment/system efficacy in vitro?

Hi, After going through literature studies, I found that in all studies focusing macrophages as target cells for inflammatory disease treatment, the cells were treated with treatment/delivery...

01 January 2016 6,074 5 View