Shivasankari Gomathinayagam

6 Questions 16 Answers 0 Followers

Questions related from Shivasankari Gomathinayagam

Protein-DNA complexes stuck on the wells of native PAGE during unwinding assay.

I am doing an unwinding assay for my protein with a fork substrate and my protein-DNA complex is stuck in the wells. My quench to stop the reaction has 0.5% SDS, unlabelled strand, EDTA and...

05 June 2017 3,827 10 View

How to prevent clumping of EBV trasnfected lymphocytes?

I am culturing lymphocytes and they tend form visible clumps.. Does anybody know how to avoid this and if this is common ? I am new in culturing them. Thanks

31 May 2016 755 7 View

Help with imaging Western blots on an imager?

I am new to using the imager for my western blots. I am not satisfied with the images I get. I would like to know how to get good images using a imager. 1) Do you pour the substrate on the top of...

30 November 2015 9,837 10 View

Which antibody could I use against BRCA2 for Immunofluorescence?

I am looking for an Antibody to use in Immunofluorescence specific for BRCA2. I am not able to find a convincing IF image of BRCA2 by IF. Lot of scientists use the tagged version of BRCA2 in their...

16 September 2015 3,920 3 View

How can I stabilise a protein-DNA complex in isolation?

I have purified my protein-Holliday junction complex by SEC, but the purified complex is not stable. The protein dissociates from the DNA. I would want the complex to be stable to perform further...

24 January 2014 6,557 2 View

Has anybody experienced protein sticking to the frits of the Superdex 200 column?

I am working on a human helicase protein and when I load them on a SEC column Superdex 200 (GE), I don't get a good recovery. In addition, when I load my protein-DNA complex into the same column...

22 January 2014 6,352 3 View