Shivansh Mahajan

3 Questions 5 Answers 0 Followers

Questions related from Shivansh Mahajan

How well does thrombin digest work in PBS buffer (pH 7.4)?

I am trying to cleave a His-tag on a protein (~60 kDa) using thrombin cleavage. Although thrombin cleavage reactions are typically carried out at pH 8.4, has anyone had success at pH 7.4? The...

12 December 2019 3,925 3 View

Should protein extracts be vacuum filtered before purification?

I have crude protein extract that I want to purify on a SP-Sepharose column. Is it okay to filter the crude sample to get rid of suspended debris by vacuum. Can the foam generated by doing so, in...

07 July 2018 3,317 5 View

Can phenylalanine do hoffmann elimination to give trans-cinnamic acid ?

Trying to know if the synthesis is possible non-enzymatically, without using Phenylalanine Ammonia Lyase (PAL).

11 November 2017 6,351 4 View