Shivakiran Makam

4 Questions 10 Answers 0 Followers

Questions related from Shivakiran Makam

Is there any colorimetric titration method to determine the quaternary ammonium ions ?

I am looking for some colorimetric reaction , wherein I should be able to determine the quantity of quaternary ammonium ions such as benzalkanium in water samples. I have a limitation of very low...

08 August 2016 6,127 7 View

Is there any method to coat Whatman filter paper with colored metal complexes similar to that of pH indicator papers?

I am working to immobilize/ fix/coat (however you call it) metal complexes such as Co, Cu derived colored complexes on to Whatman 1 filter paper for color based indicators. But whenever I wash the...

02 February 2015 154 10 View

Has anyone used GJ1158 E. coli host cell for expression of recombinant proteins?

GJ1158 host cells were originally designed by CCMB, Hyderabad, India. It is E.coli host cell containing T7 polymerase gene under the control of a salt inducible promoter, i.e., instead of IPTG,...

08 August 2012 4,082 2 View

Do proteins precipitate during dialysis?

I have purified recombinant proteins by Ni-NTA IMAC method. I had dialysed them overnight @ 4 OC to remove urea.After dialysis I found precipitation settling at the bottom of the dialysis bag...

11 November 2011 4,390 35 View