Shiv kumar Mishra

3 Questions 16 Answers 0 Followers

Questions related from Shiv kumar Mishra

How can we measure the cell density and cell seeding per well?

I have successfully established MIN6 cell Culture and now planing to do further experiments with cell viability assay and cell cytotoxicity assay. For this I am asking for guidance on protocols....

04 September 2013 7,680 10 View

Ideal quantity and % of antibiotic used in media for animal cell culture?

I just want to know whether it is necessary to add antibiotic to cell culture media?

13 August 2013 2,093 18 View

Protocol for primary cell culture establishment from normal rat pancreatic islet and how can I improve the number of generations of primary cell lines?

I want to establish primary cell culture from normal rat pancreas and want to get cell line of improved number of generations.

27 May 2013 4,841 1 View