7 Questions 14 Answers 0 Followers
Questions related from Shirley Wu
I am culturing LentiX HEK293 cells and noticed round particles in cell culture. They are about 1-3 microns in diameter, so unlikely to be mycoplasma (which are
09 January 2019 7,766 3 View
I noticed that the lentiviral infection efficiency in primary human T cells decreased very fast and much more significantly than in normal cell lines (eg., HEK, Jurkat, tumor cells lines, etc)....
13 October 2018 9,650 8 View
Most protocols say store lentivirus at -80C and some recommend snapfreeze in liquid nitrogen and then store at -80C. My question is that can you store lentivirus in liquid nitrogen? Thanks!
09 December 2017 8,271 4 View
I was doing subcutaneous injection in mice, and every time I withdraw the needle, there would be a drop of liquid leaking out. Does anyone know how to prevent that? I am using the BD Ultra-FineTM...
16 October 2017 6,184 9 View
Most studies on CAR-T cells use NSG mice (B/T cell deficient) based on the papers I found. Only a few use nude mice (T cell deficient, B cell present), which is what we are hoping to do. Does...
21 July 2017 7,801 3 View
I am using RetroNectin to coat plates for viral infection. The protocol (see the link below) says to block with 2% BSA for 30 min and wash with PBS after removing the RetroNectin coating. Does...
23 March 2017 7,699 3 View
When making lentivirus, after harvesting the virus, do you usually filter them with 0.22um or 0.45um filters? Most protocols use 0.45um, but I was wondering would 0.22um hurt? What is the size of...
18 September 2016 898 12 View
