Shirley Wu

9 Questions 15 Answers 0 Followers

Questions related from Shirley Wu

Anyone has experience with prostate tumor mouse model?

Hi, We are trying to establish subcutaneous tumor in NSG mice using prostate cancer cell line PC3 (engineered with firefly luc). We use IVIS to monitor tumor growth and noticed that the tumor...

29 June 2019 8,147 3 View

Round live (?) particles in cell culture (but not mycoplasma)?

I am culturing LentiX HEK293 cells and noticed round particles in cell culture. They are about 1-3 microns in diameter, so unlikely to be mycoplasma (which are

09 January 2019 7,817 3 View

Why lentiviral infection efficiency decreases dramatically over time in T cells?

I noticed that the lentiviral infection efficiency in primary human T cells decreased very fast and much more significantly than in normal cell lines (eg., HEK, Jurkat, tumor cells lines, etc)....

13 October 2018 9,692 8 View

What are these structures on this mouse MRI image?

Attached are two coronal MRI images of a mouse lying on its side, with the left image taken 2mm lower than the right one. Can anyone tell me if the part circled by the red line is the hindlimb of...

08 January 2018 1,075 1 View

Can you store lentivirus in liquid nitrogen?

Most protocols say store lentivirus at -80C and some recommend snapfreeze in liquid nitrogen and then store at -80C. My question is that can you store lentivirus in liquid nitrogen? Thanks!

09 December 2017 8,321 4 View

How to avoid leakage after subcutaneous injection in mice?

I was doing subcutaneous injection in mice, and every time I withdraw the needle, there would be a drop of liquid leaking out. Does anyone know how to prevent that? I am using the BD Ultra-FineTM...

16 October 2017 6,250 9 View

Can nude mice be used for CAR-T cell immunotherapy studies?

Most studies on CAR-T cells use NSG mice (B/T cell deficient) based on the papers I found. Only a few use nude mice (T cell deficient, B cell present), which is what we are hoping to do. Does...

21 July 2017 7,881 3 View

Why do we need to block and wash the RetroNectin coated plate?

I am using RetroNectin to coat plates for viral infection. The protocol (see the link below) says to block with 2% BSA for 30 min and wash with PBS after removing the RetroNectin coating. Does...

23 March 2017 7,742 3 View

Do you use 0.22um or 0.45um filter when making lentivirus?

When making lentivirus, after harvesting the virus, do you usually filter them with 0.22um or 0.45um filters? Most protocols use 0.45um, but I was wondering would 0.22um hurt? What is the size of...

18 September 2016 960 12 View