Shikha Chaudhary

3 Questions 5 Answers 0 Followers

Questions related from Shikha Chaudhary

Hey, I am working on developing ELISA assay for detection of Dengue antibodies (IgG & IgM). Can anyone help to resolve false negative results?

I have been facing the issue of False negative results in both Dengue IgG & IgM assays. I have tried various buffer compositions with commonly known blockers to resolve sample interference but...

22 December 2023 7,951 3 View

Hello, can anybody let me know the extinction coefficient for glycine at 220 nm?

I am working on Glycyl Glycine di peptidase (GGDP) enzyme activity assay, where GGDP hydrolysis glycyl glycine substrate releasing glycine as the product. I am using precipitation reagent...

19 December 2021 9,600 13 View

I have been trying to dissolve 2-naphthylamine in 2% ethanol but some particles remain undissolved. Please suggest how to dissolve it completely?

I incubated the solution in a hot water bath for 30-35 min, and also tried by adding 1:10 dilution of 1N NaOH but the particles remained undissolved. Please suggest some other solvent or method....

01 November 2021 4,002 1 View