Shiju gu

5 Questions 6 Answers 0 Followers

Questions related from Shiju gu

How to avoid the unspecific binding in live cell imaging?

Dear scientists, I am currently doing some researches on live HEK cell imaging using quantum-dots. I basically pre-conjugate a biotinylated-toxin to streptavidin-Quantum-dots (ratio is 3:1), and...

23 March 2021 3,943 2 View

How to know exposure time of SU-8 5 and SU8 100?

Hello, dear friends I want to make microfluidics channels by following "Taylor, Anne M., et al", basically on page 2 and page 8(in the attach), talks about how to make it. Basically a Su-8 5 is...

22 January 2019 9,593 10 View

How to get ride of PDMS residue in the microchannels?

I made some microchannels(50um wide, 3mm long and around 80um deep) by using SU8 2025 and silicon wafer, and then pour some PDMS and desiccate them for about 3 hours and them heat at around 120...

30 July 2018 957 4 View

What is size of a rat hippocampus cell?

I am trying to building some microchannel devices currently to culture neurons, but I want to know the initial size(diameter) of the rat hippocampus cells (before they formed the axon and...

21 December 2017 4,897 3 View

SU8 2025 detached from the wafer, how to solve that?

i am trying to make some PDMS channel like 10um and 20um and 50um, but when I try to make the SU8 pattern, the 10um detached from wafer and messed up, while the 20um and 50um looks pretty fine,...

21 November 2017 447 7 View