Shezaib Siddiqui

5 Questions 7 Answers 0 Followers

Questions related from Shezaib Siddiqui

Why electroblotter electrodes rusted after overnight electroblotting (wet transfer)?

I setup an electro blotting experiment for overnight wet transfer using Tris glycine based transfer buffer at 20V. Next day , to my surprise there was lots of rusty froth in the tank. Also their...

17 August 2021 4,331 2 View

What kind of Enzyme Inhibition is it?

I am working on beta glucuronidase and found a completely new class of potent inhibitors as revealed from their IC 50 values. however, I am having difficulty in establishing kinetics for these...

07 June 2017 613 8 View

How to switch my research domain?

I have experience in molecular biology, specifically cloning, expression and purification of bacterial proteins, but my interests are shifting towards stem cells, Immunology and autologous...

10 April 2017 4,876 2 View

What are the good tools for Protein Structure and function prediction?

I am working on proteins designated as "Hypothetical Proteins". I would like to know about some good tools for structure and function prediction.

29 January 2016 9,961 8 View

How can I prevent protein dimerization?

I have cloned, expressed, and purified an hypothetical protein (13 KDa) from S. aureus using pET 25b expression vector. Protein contains 1 cysteine residue which may accounts for dimerization. To...

26 May 2014 2,624 3 View