Shen Wang

9 Questions 8 Answers 0 Followers

Questions related from Shen Wang

Is the protein (or protein complex) thermostability in tropical species higher than those living in arctic?

Hi, I am wondering whether the protein/protein complex thermostability in tropical species is higher than those living in the arctic. If it is valid, is there any relevant evolutionary theories? I...

13 July 2020 1,285 0 View

What is the optimal SUV/LUV concentration for supported lipid bilayer (SLB) formation?

Hi everyone, I am trying to make SLB by liposome rupture, recently i found the SLB did form, however, after bleaching the hole could not completely recover. I think there exists multi-layers. For...

01 July 2019 5,028 2 View

Can one calculate the translational diffusion coefficient from fluorescence anisotropy?

Hi, Time-resolved fluorescence anisotropy is used for analyzing rotational diffusion. So my question is, can one measure the translational diffusion by fluorescence anisotropy (steady-state or...

07 January 2019 8,135 3 View

How to optimize the expression of a protein containing a centered transmembrane helix in bacterial system?

Hi, everyone! I have a single-pass transmembrane protein (its functional domain is in cell lumen). Now i want to link a fluorescent protein (e.g. EGFP or dsRed) to its C-terminus. The chimeric...

14 August 2018 2,790 9 View

Can anyone give some instructions for the in vitro yeast Atg8 lipidation assays?

Hi, I ve been stuck by the in vitro yeast Atg8 lipidation assays for a long time. I followed the basic protocol presented by Ohsumi's group [ Ichimura, Y. et al, (2004). In vivo and in vitro...

20 June 2018 4,727 0 View

Why the fluorescence correlation spectroscopy (FCS) measurement recommends a water-immersed objective?

Is there any problems to use an air or oil-immersed objective? Thanks for all of your advice.

24 November 2017 7,397 2 View

Are there any theoretical and practical guide for fluorescence-based single particle/molecule tracking method?

Greetings to everyone here! I am new to this field. Actually I've read some papers about fluorescence-based single molecule tracking, but I got confused that their methods differ from each other....

24 November 2017 2,709 3 View

Can one edit the genome of the yeast haploid using homologous recombination?

I am amateur in yeast genomics, but recently i am trying to screen some ts mutants of yeast. Most of the literatures that i ve reviewed described methods using diploid shuffle or plasmid shuffle...

08 August 2016 2,918 3 View

How do membrane binding proteins affect lipid translational diffusion?

For instance, transmambrane proteins, peripheral membrane protein and lipid bilayer.

01 January 1970 2,737 4 View