Shekhar Patil

2 Questions 3 Answers 0 Followers

Questions related from Shekhar Patil

Did anyone used pLV-tetO plasmid for any of your experiment related to lentivirus transduction?

I want to know the exact map of this lentivirus plasmid if someone used this plasmid i just want to know the exact location of the 3'LTR within this plasmid.

28 December 2022 4,249 0 View

Can I use Zeocin for selection to select stably transgene expressing mES cells ?

I am using lentiviruses to transfer my gene into the mES cells and the zeocine resistance gene is present outside of the 5'LTR and 3'LTR region of the transfer plasmid. so to select stably...

22 December 2022 8,532 3 View