Shehan Kaleel

2 Questions 2 Answers 0 Followers

Questions related from Shehan Kaleel

Does different HPLC's give different peak areas?

Hello, we recently acquired a shimadzu HPLC and started our analysis through it. What I noticed usually is the peak area of our samples are very different compared to the results got from our...

04 December 2020 696 4 View

What causes peak separation (or resolution) to decrease suddenly in a HPLC chromatogram?

Hello, I've been running Budesonide in my HPLC system and I was getting my spectrum with the separation of the two epimers as two separate peaks. But since a few days back the two peaks have poor...

11 June 2020 4,449 3 View