Sharon Miracle Nayagam

3 Questions 3 Answers 0 Followers

Questions related from Sharon Miracle Nayagam

Will CTAB buffer dissolve acetone-precipitated proteins?

I tried extracting my cartilaginous tissue sample with the help of RIPA buffer, precipitated it with the help of acetone: methanol (8:1) volume to volume of sample. Tried dissolving it with CTAB...

27 June 2021 7,035 1 View

Hi Can anyone help me out in getting neat SDS PAGE profile??

My sample of interest is highly fibrocartillaginous in nature. I need to extract total protein content from it for which i use RIPA buffer (50mM Tris PH7.4, 150mM Nacl, 0.1% SDS, 0.5% SDC, 1%...

22 July 2019 8,371 7 View

Which form of protein is highly stable? Raw tissue or extracted form??

My sample of interest is cartilaginous tissues which has lot of glycans and lipids. Denaturants and salts like SDS, GHCl, Nacl, SDC were used to extract the entire protein content from tissue...

01 January 1970 3,902 0 View