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Questions related from Sharon Miracle Nayagam
I tried extracting my cartilaginous tissue sample with the help of RIPA buffer, precipitated it with the help of acetone: methanol (8:1) volume to volume of sample. Tried dissolving it with CTAB...
27 June 2021 7,027 1 View
My sample of interest is highly fibrocartillaginous in nature. I need to extract total protein content from it for which i use RIPA buffer (50mM Tris PH7.4, 150mM Nacl, 0.1% SDS, 0.5% SDC, 1%...
22 July 2019 8,363 7 View
My sample of interest is cartilaginous tissues which has lot of glycans and lipids. Denaturants and salts like SDS, GHCl, Nacl, SDC were used to extract the entire protein content from tissue...
01 January 1970 3,894 0 View