Sharif Moradi

5 Questions 18 Answers 0 Followers

Questions related from Sharif Moradi

How many mRNA targets mediate the effects of a single miRNA? A single target?

Hi there, It has been a long time that I think about one of the important aspects of miRNA research: how many genes mediate miRNA effects? According to the literature, most if not all of miRNAs...

04 August 2017 9,741 6 View

What is the easiest way to analyze the DNA methylation state of an imprinted locus?

We are going to determine the methylation status of an imprinted locus in mouse embryonic stem (ES) cells cultured under three different conditions (that are likely to modulate the methylation of...

07 February 2017 1,921 4 View

Is there any iPS kit or OSKM-containing MEFs inducible by DOX?

I'm going to analyze the effect of a couple of genes/agents on the efficiency of iPSC generation. To save time, I am looking for any reliable iPS kit or OSKM-containing MEFs inducible by adding...

06 May 2015 3,702 2 View

Is there anyone with expert advice on IPSC derivation from MEFs using episomal vectors?

I've been recently trying to derive iPSCs from low-passage MEFs using episomal vector electroporation (3 vectors: hOCT4-p53shRNA; hSOX2/hKLF4; and hN-MYC/hLIN28 - (h=human)). Because I need to...

13 February 2015 5,092 13 View

What is the minimal cell number per sample for cell cycle profiling?

Does anyone of you know how many cells is needed to run a cell cycle distribution analysis using flow cytometry (PI staining)? Mouse ES cells have been treated with a certain agent and I expect...

24 January 2015 9,039 14 View