Sharif M. Ridwan

4 Questions 8 Answers 0 Followers

Questions related from Sharif M. Ridwan

Is it okay to store slides in refrigerator overnight after heat induced antigen retrieval, if I need to stop for the day?

Hello, I am doing heat induced antigen retrieval for fixed frozen sections (mouse bones). Sometimes I need to stop for the day following ag retrieval. I was wondering if is okay to store slides in...

29 December 2023 3,538 0 View

Is permeabilization step a must for intracellular antigens while using 7-10 um frozen sections of brain for IF?

I am using 7-10 um frozen sections of mouse brain for IF. My target antigens are both intracellular (trying to determine) and membranous. Do I need to use any permeabilizer ? If so, which one...

10 April 2018 5,042 6 View

Can anyone suggest how much DMSO can be given to mouse by oral gavage without causing any harm?

I need to dissolve Vardenafil HCL in DMSO (Solbility 2mg/ml DMSO) in order to administer in a mouse (20mg) via oral gavage. That means I have to administer Vardenafil in almost 100 micro gram of...

02 December 2016 7,447 2 View

Can anyone suggest: The post fixing of the whole mouse brain in 4% formaldehyde - how long should the procedure continue?

I was trying to post fix the whole mouse brain in 4% formaldehyde and I was wondering what is the best time period I should suspend the whole brain in the solution so that the whole tissue gets...

01 January 1970 145 8 View