Shams Saad

3 Questions 2 Answers 0 Followers

Questions related from Shams Saad

What is the overall protein content in one mouse cardiomyocyte?

03 October 2023 9,208 1 View

What are the best culture conditions for keeping mouse oocytes healthy and arrested at prophase I for 48 hrs after microinjection?

I have been trying to optimize the culture conditions for siRNA-injected prophase I mouse oocytes (CF-1) for 48 hrs. I have been culturing them in Bicarb with 250 µM dbcAMP and I change with fresh...

18 September 2023 8,948 0 View

Why do I keep getting these weird ladder bands on my SDS-PAGE gels?

I am using 8% gel and Bio-Rad Precision Plus Protein Dual Color Standards. I have been loading 15ul of my ladder, and when I decreased the loaded amount to 5ul, I got the same weird appearance.

26 October 2022 7,634 3 View