Shalom Sara Thomas

6 Questions 5 Answers 0 Followers

Questions related from Shalom Sara Thomas

Preparation of sample for gas chromatography analysis of saturated and unsaturated fatty acids, can any experts answer some of my doubts?

Hello, I am doing the sample preparation for gas chromatography for the first time. Following a protocol available in the lab, but I am facing some difficulties. The labmates who were experts in...

15 April 2021 1,950 3 View

Can you please help me in this conversion of liver lipid in mM to mg/g liver?

I am using an enzymatic kit for Lipid analysis from liver tissue and I have trouble in converting the 0.018 mM of TG to mg/g of liver. Please help me with this. The details of the experiments are...

20 March 2021 4,431 5 View

3T3-L1 cells, is this a bacterial contamination or a cross contamination with different cell line? or something else?

Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...

14 February 2021 4,277 3 View

What solvent should I use to dissolve Mosapride citrate for in vivo studies?

Hello, Can you please give your suggestions to dissolve Mosapride citrate (a gastric emptying agent) for oral gavaging in mice? This chemical dissolves in DMSO. However, using a high...

14 July 2020 4,592 3 View

Can someone please help me with the dose calculation in animals?

Hello, I wanted to know the method of calculating the converting the concentration of a sample in gram/100 gram diet to oral administration concentration in mg/kg body weight of mice. For example,...

09 January 2020 8,493 2 View

Can I use Tris- hydrochloride instead of TRIS?

Hi, I am preparing STE buffer for genotyping in the method following. I want the buffer to have a final pH of 8. Tris - 100mM EDTA- 5mM SDS-0.2% NaCl- 200mM Is TRIZMA base is more suitable or...

24 January 2019 5,064 2 View