Shaloam Dasari

5 Questions 8 Answers 0 Followers

Questions related from Shaloam Dasari

Need to get transcriptome of a protein of interest, What is the general protocol for cDNA microarray ?

Have to find out expression of a protein of interest, using cDNA microarray. Help me with a general protocol how to carry out this experiment

20 February 2017 3,497 3 View

Protein bands were not transferred from gel onto PVDF membrane. Any suggestion for better transfer?

Hi, USed ANy KD gel from Biorad. Gel run was good. Transfer was not efficient, bands were still appearing on the gel but with low color intensity. I used TGS to transfer (20% methanol), 60V for 90min.

02 August 2016 6,883 5 View

What is the relatioship between lipid peroxidation and antioxidant enzymes in case of treated Vs normal cancer cells ?

I know that there exists a direct relationship between lipid peroxidation and SOD, Cat, GPX etc., But there is lot of ambiguity when it comes to working with a particular cell line. Please post...

20 April 2015 5,132 7 View

Do you have any suggestion on online database for mitochondrial proteins in HL60 cells?

I work with HL60 cells. I would like to know the online database for mitochondria proteins in this particular cell lines. So that I can pick certain proteins and start working. Thank you.

03 March 2015 560 2 View

Can anybody help me with a kit that estimates mitochondrial protein levels ?

The mitochondria is the primary target for oxidative stress induced by Cisplatin, resulting in loss of mitochondrial protein sulfhydryl group. We have some interesting data on Oxidative stress...

03 March 2015 3,590 14 View