Shalini Dubey

4 Questions 8 Answers 0 Followers

Questions related from Shalini Dubey

Can I use synthetic spike-ins for data normalization for plasma miRNA qPCR?

I am working with circulatory miRNAs derived from plasma. My study involves 4 case groups and 1 control group. I have tested the utility of 3 miRNAs as endogenous controls and none of them have...

01 January 2020 8,969 4 View

CDNA quantification needed?

I am working with microRNAs from human plasma samples. I have normalized the initial input concentration of the extracted microRNAs for the reverse-transcription step using Qubit. I would like to...

07 July 2019 5,623 5 View

Can anyone help me understanding why am I getting jagged amplification curve for my miRNAs in plasma sample?

I have used mircury exosome serum/plasma kit along with trizol for extraction of my miRNAs from human plasma. RNA concentration was 623.8ng on nanodrop with 260/280 ratio of 2.12. For RT-PCR and...

06 June 2019 3,615 5 View

Can anyone provide a reliable protocol with tips and tricks for miRNA isolation from human plasma using Trizol?

Trizol protocol I am using, provides good total RNA concentration and quality but the miRNA spike (Cel-miR39) is not being amplified in qPCR. Starting volume: 600microlitre Plasma Elution:...

06 June 2019 2,473 10 View