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I am using 1% Ga cacodylate-filtered buffer for quick hard fix of my phage sample to grid then perform negative staining by 2% uranyl acetate. But the phage picture look like empty. Does it mean...
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Normally I use Minisart, 0.22-μm pore size filter for phage isolation from E. coli. But sometimes it is so difficult to filtrate the phage. It needs a lot of pressure and it hurts my hand. Is...
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