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Questions related from Shakhinur Islam Mondal
I am using 1% Ga cacodylate-filtered buffer for quick hard fix of my phage sample to grid then perform negative staining by 2% uranyl acetate. But the phage picture look like empty. Does it mean...
30 August 2016 3,198 4 View
I am trying to localize phage head proteins by immunogold labelling. I found some structures like the gold particle bound around the phage head but phage structure is not clear. It seems to be...
23 July 2015 4,837 10 View
Normally I use Minisart, 0.22-μm pore size filter for phage isolation from E. coli. But sometimes it is so difficult to filtrate the phage. It needs a lot of pressure and it hurts my hand. Is...
12 November 2013 478 6 View
Generally I gave PEG treatment for 1 hour in 40C. It works fine. But for my work schedule it is better to use O/N PEG treatment. Is there any toxic or other effect of PEG which decreases the...
12 November 2013 5,278 2 View
