Shailesh Nair

4 Questions 13 Answers 0 Followers

Questions related from Shailesh Nair

How to annote bacteriophage genome and identify the bacteriophage?

I have sequenced a bacteriophage (after CsCl purification and DNA extraction) and assembled the paired ends. The assembled .contig file is a multi FASTA file with many nucleotide sequences (of...

11 November 2019 357 4 View

Is there any efficient selective media/technique for Culturing Rhizobium species on agar plates?

A 16s amplicon sequencing from mixed bacterial-algal culture system showed moderate rhizobium reads ( abundant bacteria - 55%, other moderate bacterial reads ranged from 2% - 30%, Rhizobium...

06 June 2019 4,235 7 View

Is it possible to segregate and identify algae and bacterial transcriptome and exoproteome saperately after sequencing a co-culture system?

I optate to study bacterial-Synechococcus transcriptome and exoproteome in a co-culture system. I can either seperate the two organisms by using appropriate size filters and sequence them...

05 May 2019 8,646 5 View

Which is the best/optimum preservative techniques for RNA preservation?

we want to carry out transcriptomics study of a mixed marine bacterial community. The experiment is designed as such that we have to preserve the samples for later transcriptomic studies...

02 February 2019 9,188 18 View