Shahrooz Nasrollahi Shirazi

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Questions related from Shahrooz Nasrollahi Shirazi

How can IL-10 expression in B cells be effectively induced?

We are conducting flow cytometry to detect IL-10 secretion in B cells, specifically regulatory B cells (Bregs). For this purpose, we utilize either whole blood (100 μl) or isolated PBMCs (1...

21 May 2023 2,191 1 View

What is the most promising method in your opinion to detect the cAMP levels directly?

Before, I was incubating the cells with [3h]Adenine overnight and thereafter separating cAMP (from ATP,ADP and AMP) by sequential chromatography (using Dowex and alumina columns) . This method...

03 May 2017 1,586 4 View