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I am looking for a protocol for transfection of insect cells with in vitro transcriptions using Lipofectamine 2000. I tried the protocol recommended by Thermo Fisher but NO success. Any suggestion?
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I detected several new viruses in my target insect through NGS and RT-PCR. Now I would like to use S2 cells to see if I can extract infectious viruses from these infected field insects. I read...
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