Shahid Ali

9 Questions 13 Answers 0 Followers

Questions related from Shahid Ali

Can someone tell me , which vector and strain is best to express Thermomyces lanuginosus lipase in E. coli system?

Actually , i want to express Thermomyces lanuginosus lipase (TLL) in E coli system. so please help me and if somebody have protocol/procedure plz share with me. Thanks in advance!

05 May 2019 9,647 3 View

How can i place a protein in the center of prepared box with different solvents ?

ACtually ,i want to simulate a protein with different types of solvent present like Glycerol+urea+chloride. I created a box with these mix solvents. Now , i am facing the problem is that : how...

01 January 2019 2,766 6 View

How can i calculate number of chloroform molecules in 50% v/v conc. ?

Actually, i want to check effect of chloroform on my enzyme,so i am going for MD simulation with chloroform.But problem is that, i dont know how to calculate number of molecules in 50% v/v conc....

11 November 2017 3,632 5 View

How can i install all packages of Amber 14 (MD simulation) ??

actually ,i want to learn MD simulation on Amber 14 .but i cannot understand ,how to install tLeap etc.and i am confused .so can anybody tell me....easy way to learn that software and please...

09 September 2017 9,535 2 View

How to compile trj_cavity module in gromacs version 5.X on cluster server ??

How to compile trj_cavity module in gromacs version 5.X on cluster server ?? actually ,i want to determine cavities and their volume with respect to time . so, i am trying to install trj_cavity...

04 April 2017 3,183 2 View

Can i use Rosetta Design for mutants design of enzyme ??

actually ,I am working on enzyme design by computational methods ,and i want improve enzyme activity and stability ,so can i used some other tools ?? thanks !!

02 February 2017 4,428 4 View

How to make topology file for metalloenzymes (Vanadium dependent chloroperoxidase).I tried by Prodrug server but it fail ?

Prodrug result : PRODRG> Starting up PRODRG version AA100323.0717 PRODRG> Parameter set 'pd/gromos96' (fftype=2). PRODRG> MOL mode detected. PRODRG> Found unsupported atom V in...

11 November 2016 3,181 3 View

Which software is most significant for the protein modeling ??

01 January 2016 9,827 0 View

Is there any online server available for virtual screening of inhibitors against an enzyme ??

and how to validation by insilico methods??

01 January 1970 2,460 3 View