Shahid Shahid

3 Questions 13 Answers 0 Followers

Questions related from Shahid Shahid

I am trying to purify a cytochrome enzyme and put 10His tag at the C terminal region. It is not binding to the Ni column, would you help me ?

I am using Swanella bacteria (gram negative) to purify cytochrome (a periplasmic protein, C type heme, homodimer). I put 10 Histag at the C terminal region, because N terminal region have...

09 May 2019 1,014 17 View

How can I purify C terminal His tag protein ?

Hi I am stuck this purification step since last one year my periplasmic protein ccNiR have leading sequence at N terminal, so I added 10 His tag at C terminal. I used FPLC and Ni column to purify...

23 May 2018 5,898 3 View

How can I separate LC3A/B I and II bands on western blot?

I am facing difficulty to show separate bands for LC3A/B bands. I find only one band. Here are my procedures; I cultivated THP-1 (monocyte, macrophage both tried) cells with 2% FBS Media 24...

01 January 1970 9,816 4 View