Shahid Aziz

2 Questions 1 Answers 0 Followers

Questions related from Shahid Aziz

How can I re-dissolve the protein pellet or How can I resolubilise the dried protein serum samples?

I have a resolubilisation problem with serum samples. 250µl serum was 500µl lysis buffer (8M Urea, 100mM TrisBase, 2% w/v SDS) and centrifuged at 16.000 x g for centrifuged at 16,000 x g for 5min....

04 August 2021 3,629 4 View

Can someone please share the protein extraction protocol using SDS, Urea, Tris base and TCEP lysis buffer?

I have been working on the early detection of a gastric cancer biomarker in gastric disease patients. And facing difficulties in protein extraction from serum samples.

16 March 2021 7,281 0 View