Shahid Aziz

6 Questions 1 Answers 0 Followers

Questions related from Shahid Aziz

How can I re-dissolve the protein pellet or How can I resolubilise the dried protein serum samples?

I have a resolubilisation problem with serum samples. 250µl serum was 500µl lysis buffer (8M Urea, 100mM TrisBase, 2% w/v SDS) and centrifuged at 16.000 x g for centrifuged at 16,000 x g for 5min....

04 August 2021 3,634 4 View

Can someone please share the protein extraction protocol using SDS, Urea, Tris base and TCEP lysis buffer?

I have been working on the early detection of a gastric cancer biomarker in gastric disease patients. And facing difficulties in protein extraction from serum samples.

16 March 2021 7,286 0 View

Can we use T-PER Tissue Protein Extraction Reagent (25mM bicine, 150mM sodium chloride; pH 7.6) to extract total protein from serum samples??

I am using T-PER Tissue Protein Extraction Reagent (contains a proprietary detergent in 25mM bicine, 150mM sodium chloride; pH 7.6) for protein extraction from gastric biopsies and lyophilizing...

16 March 2021 2,740 1 View

Why positive phi angles less common than negative Phi angles in proteins contain L amino acid??

Protein folding is accelerated by constraints placed upon the folding proteins that restrict the number of possible conformations that are sampled during the folding process. One such limitation...

12 December 2020 6,737 2 View

Can we extract DNA from biopsy debris after treating with T-PER Tissue Protein Extraction Reagent?

I am working on virulence factors and proteins associated with H. pylori infection. For this, I have collected gastric biopsies and treated them with T-PER Tissue Protein Extraction Reagent (...

07 December 2020 3,675 2 View

How can we detect VOCs in exhaled breath sample using ISQ 7000 single quadrupole GC-MS system??

The ISQ 7000 single quadrupole GC-MS system contained an injection port for analysis of liquid samples, but I want to detect VOCs in exhaled breath samples of patients having different...

03 December 2020 4,851 5 View