Shahd Abu Kamel

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Questions related from Shahd Abu Kamel

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

07 August 2024 758 3 View

What protocol is optimal for extracting RNA from frozen tumor tissue for Next Generation Sequencing (NGS)?

hello everyone , I'm currently working on extracting RNA from frozen tumor tissue that was snap-frozen in liquid nitrogen and stored at -80°C. I've attempted a protocol involving cutting a small...

15 March 2024 8,782 0 View