Shahd Abu Kamel

3 Questions 5 Answers 0 Followers

Questions related from Shahd Abu Kamel

Should I re-select transfected cells with puromycin before doxycycline induction?

Hi, I transfected adherent cells with a plasmid containing a Tet-on/off system and GFP. GFP and my protein of interest are separated by a P2A sequence. The cells were selected with puromycin for...

21 April 2025 5,788 7 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

07 August 2024 1,734 3 View

What protocol is optimal for extracting RNA from frozen tumor tissue for Next Generation Sequencing (NGS)?

hello everyone , I'm currently working on extracting RNA from frozen tumor tissue that was snap-frozen in liquid nitrogen and stored at -80°C. I've attempted a protocol involving cutting a small...

15 March 2024 8,882 0 View