Shahan Syed

2 Questions 5 Answers 0 Followers

Questions related from Shahan Syed

Can ligand-binding assays be done with fixed cell samples?

Dear colleagues, we are interested to study binding of a protein molecule on endothelial cells. The cells are treated with bacterial supe, which cleaves off receptors on endothelial cells. The...

02 November 2017 6,955 3 View

Can heterologous expression (of membrane proteins) perturb their integration into the membrane?

I have expressed a human membrane protein complex in insect cells (Sf21 cells) using MultiBac expression system. At time of expression the insect cells appeared to be old- but were still growing...

04 July 2015 747 2 View