Shafqat Ali Khan

2 Questions 12 Answers 0 Followers

Questions related from Shafqat Ali Khan

How to insert a reporter gene using CRISPR/Cas9 method?

I am working with human ES cells and interested in inserting a GFP and RFP reporter downstream to both the alleles of my gene of interest to monitor its expression. I am thinking to keep 1 kb...

30 March 2016 8,031 11 View

Can we fix and store ES/iPS cells instead of immediately processing them for FACS analysis?

I have regularly fixed cancer cells in 70% chilled ethanol and stored them at -20 degree and processed them for PI based cell cycle analysis afterward. However, I don't know if this can be done...

11 March 2016 3,602 4 View