Shachar Raz

2 Questions 5 Answers 0 Followers

Questions related from Shachar Raz

Is there a good protocol for extraction of tRNA with amino acid using TRIzol?

Hello everyone, I would like to extract tRNA using TRIzol (from ambion) and to get the methionine-tRNA (with the amino acid attached). I know that tRNA is extracted using the TRIzol reagent but am...

10 February 2016 3,623 4 View

Has anyone encountered different results when extracting a membrane protein using a hypotonic buffer and NP-40 or a needle-based lysis?

We recently encountered a weird difference between two extraction methods that should yield the same results, and we could not find an explanation: 1. Cell lysis was performed using a hypotonic...

14 December 2014 7,503 4 View