Settanan Plangsiri

6 Questions 10 Answers 0 Followers

Questions related from Settanan Plangsiri

MRNA synthesis and purification, T7 MEGAscript Transcription Kit troubleshooting?

Hello everyone! I am currently working on a project that involves the use of mRNA. I started off my ordering plasmid from VectorBuilder (which contains T7 Promoter, gene-of-interest, Poly-A-tail...

19 December 2023 1,143 4 View

Why are 293 HEK cell used as viral vector production cell over other cell lines? What advantage does it have?

I am perplexed as to why HEK cells are so widely used as a viral vector production cell line? (To the point that there are even further specialized cell lines developed based on HEK, like for...

04 December 2022 390 3 View

Is there a way to find repressor/inhibition factor for a gene?

Is there a way to find repressor/inhibition factor for a gene? Is there a website that list them? I found it to be much harder to find compare to transcription factors... Thank you!!

11 May 2022 8,064 4 View

How do I find/select transcription factor for a certain gene?

I've got a list of genes that I would like to find transcription factor for. But when I looked the gene up on Genecard, there are so many to choose from (on some, the list goes up to 100s of...

27 April 2022 2,819 3 View

Is it possible to Reassociate Callus that has been separated by Cell Suspension Culture in plant?

When you filter out or centrifuge down the cells during cell suspension culture protocol, is there any method to reassociate them back into one callus? What would happen if I just plated them all...

13 April 2021 3,546 7 View

Can two Callus from tissue culture be combined?

I am wondering whether it is possible for two callus to be combined into one... both between genetically similar plants and genetically different plants.

12 April 2021 6,387 12 View